Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, 500 Assay Points
HTRF Human Phospho-TYK2 (Tyr1054/1055) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF SARS-CoV2 Spike S1 Protein Detection Kit, 50,000 Assay Points
HTRF SARS-CoV2 Spike S1 Protein Detection Kit, 50,000 Assay Points
Molecular Devices Excitation Filter, 535 nm, 25 nm Bandwidth
This excitation filter provides a 535 nm center wavelength with a 25 nm full-width bandwidth for fluorescence excitation in microplate assays. It is intended for use with multi-mode fluorescence microplate readers to enable selective excitation of fluorophores around 535 nm.
- Center wavelength 535 nm.
- Bandwidth 25 nm full width.
- Excitation filter for fluorescence detection.
- Compatible with multi-mode microplate and ELISA readers.
- Designed for microplate-based fluorescence assays.
Revvity Health Sciences Inc HTRF AAV2 Detection Kit, 10,000 Assay Points
HTRF AAV2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Progranulin Detection Kit, 10,000 Assay Points
HTRF Human Progranulin Detection Kit, 10,000 Assay Points
Electron Microscopy Sciences Nagoette Double Counting Cell 0.5 mm
Counting cells are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting. All complete with case and 1 pair of cover glasses. The depth of the Nageotte counting is 0.5 mm. For Liquids in Poor content.
Alkali Scientific ASI™ 6-Well Confocal Plate (35m), Clear, Adhesion Type, Sterile, 4/Cs, PS/Flux, Treated (Positive-Charged/ Surface Coated), 50/Cs, 22 x 22 x 0.13mm, Hole 3.14mm
ASI™ confocal products allow researchers to acquire high resolution microscopic images of cells in 35 mm culture dishes or 6 well plates. Low auto-fluorescence glass and FLux were used, ideal for uses of these products are confocal microscopy, phase contrast microscopy, live cell imaging and micro-manipulations. Surface materials for confocal products: Glass: naturally charged, good growth surface for adherent cellsFLux: surface modified plastic film (188 μm thick), optimal for confocal microscopy ▪ Bottom materials: Glass/FLux (no surface treatment for glass bottom)▪ Dish color: Clear▪ Dish size: 35 Ø▪ Plate size: 6well Plate▪ Hole size: 13 Ø / 20 Ø / 25Ø▪ Non – pyrogenic▪ Non – cytotoxic▪ DNase / RNase – free▪ Human DNA – free
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC5 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total HDAC5 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Phospho-VAV1 (Tyr174) Detection Kit, 10,000 Assay Points
HTRF Phospho-VAV1 (Tyr174) Detection Kit, 10,000 Assay Points